Assertion: Gram negative bacteria do not retain the stain when washed with alcohol.
Reason: The outer face of the outer membrane of Gram negative bacteria contains lipopolysaccharides, a part of which is integrated into the membrane lipids.
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Assertion: True — Gram-negative bacteria do not retain the crystal violet stain after alcohol washing; they get decolorized and later take the counterstain.
Reason: True — Gram-negative bacteria have an outer membrane whose outer face contains lipopolysaccharides (LPS). Alcohol disrupts this lipid-rich outer membrane, contributing to loss of the primary stain.
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